来自德国马克斯·普朗克生物化学研究所染色体生物学的Wolfgang Zachariae实验室发现有丝分裂的前期阶段需要受到有丝分裂特异性APC/CAma1调节的M阶段调节因子的蛋白质水解作用,本篇文章刊登在最新一期《CELL》杂志上。

-2012年10月26日《细胞》


中文翻译


【题目】有丝分裂的前期阶段需要M阶段调节子的蛋白质水解作用

【译文】虽然在DNA复制不久增殖的细胞进入M阶段,但减数分裂的首个M阶段是通过一个扩展的前期进行的,在这个扩展前期阶段同源染色体经过了重组过程。前期I的出口是通过重组检测点(RC)来控制的,在此检测点,酵母中抑制减数分裂特异性转录因子Ndt80,而这个转录因子对于B型细胞周期蛋白和其他M阶段调节因子是必须的。我们发现这个扩展的前期间断I需要额外的潜在的有丝分裂细胞周期控制的抑制,而有丝分裂细胞周期控制是由后期促进复合物(APC/C)和它的减数分裂特异性激活因子Ama1所调节的,这些复合物和激活因子可以引起M阶段调节子和Ndd1的降解作用(一种有丝分裂转录因子的亚基)。ama1Δ型突变体过早的从前期阶段I脱离并不依赖于RC,这导致重组缺陷和染色质的错误分离。因此,减数分裂机制对前期阶段I的控制依赖于选择性的有丝分裂的抑制作用,而后者是由APC/C的一个有丝分裂特异性形式所控制的。

英文原稿


[Title]Meiotic Prophase Requires Proteolysis of M Phase Regulators Mediated by the Meiosis-Specific APC/CAma1

[Authors]Elwy Okaz, Orlando Argüello-Miranda, Aliona Bogdanova, P.K. Vinod, Jesse J. Lipp, Zuzana Markova, Ievgeniia Zagoriy, Bela Novak, Wolfgang Zachariae

[Abstract]Whereas proliferating cells enter M phase shortly after DNA replication, the first M phase of meiosis is preceded by an extended prophase in which homologous chromosomes undergo recombination. Exit from prophase I is controlled by the recombination checkpoint (RC), which, in yeast, represses the meiosis-specific transcription factor Ndt80 required for the expression of B-type cyclins and other M phase regulators. We show that an extended prophase I additionally requires the suppression of latent, mitotic cell-cycle controls by the anaphase-promoting complex (APC/C) and its meiosis-specific activator Ama1, which trigger the degradation of M phase regulators and Ndd1, a subunit of a mitotic transcription factor. ama1Δ mutants exit from prophase I prematurely and independently of the RC, which results in recombination defects and chromosome missegregation. Thus, control of prophase I by meiotic mechanisms depends on the suppression of the alternative, mitotic mechanisms by a meiosis-specific form of the APC/C.

原文地址

http://www.cell.com/abstract/S0092-8674(12)01180-4

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