nature20121101-9

来自加州大学圣地亚哥分校的生物学家们解开了一种人类基因的抗病毒机制,或可解释相比其他人,一些HIV感染者的血液中具有更高数量病毒的原因。

-2012年11月1日《自然》


中文翻译


【题目】研究揭示某些HIV阳性患者体内有更多病毒的原因

【译文】哺乳动物中,病毒感染最明显的后果之一就是诱导机体免疫系统分泌I型干扰素、具有潜在抗病毒活性的细胞因子。Schlafen (Slfn)基因是一类干扰素诱导的早期反应基因(ISGs),这些基因也可被病原体通过干扰素调控因子3(IRF3)通路直接诱导。然而,许多ISGs仍旧未知或对其功能还不完全了解。本研究表明人SLFN11潜在地并特异性地遏制逆转录病毒的产生,如人免疫缺陷病毒1(HIV-1)。我们的研究揭示了SLFN11对包括逆转录、整合和转录的逆转录病毒感染周期早期阶段没有影响。SLFN11通过一种密码子使用依赖的方式选择性抑制病毒蛋白,在病毒产生后期发挥作用。进一步研究发现SLFN11结合转移RNA,并中和了由HIV引起的tRNA池的变化。我们的研究鉴定了固有免疫反应中一种新的抗病毒机制,即SLFN11在HIV感染的细胞中通过密码子偏爱差异选择性抑制病毒蛋白合成。

英文原稿


[Title]: Codon-usage-based inhibition of HIV protein synthesis by human schlafen 11

[Authors]:Manqing Li,1 Elaine Kao,1 Xia Gao,1 Hilary Sandig,1 Kirsten Limmer,1 Mariana Pavon-Eternod,2 Thomas E. Jones,2 Sebastien Landry,3 Tao Pan,2 Matthew D. Weitzman3 & Michael David1, 4

[Abstract]:In mammals, one of the most pronounced consequences of viral infection is the induction of type I interferons, cytokines with potent antiviral activity. Schlafen (Slfn) genes are a subset of interferon-stimulated early response genes (ISGs) that are also induced directly by pathogens via the interferon regulatory factor 3 (IRF3) pathway. However, many ISGs are of unknown or incompletely understood function. Here we show that human SLFN11 potently and specifically abrogates the production of retroviruses such as human immunodeficiency virus 1 (HIV-1). Our study revealed that SLFN11 has no effect on the early steps of the retroviral infection cycle, including reverse transcription, integration and transcription. Rather, SLFN11 acts at the late stage of virus production by selectively inhibiting the expression of viral proteins in a codon-usage-dependent manner. We further find that SLFN11 binds transfer RNA, and counteracts changes in the tRNA pool elicited by the presence of HIV. Our studies identified a novel antiviral mechanism within the innate immune response, in which SLFN11 selectively inhibits viral protein synthesis in HIV-infected cells by means of codon-bias discrimination.

原文地址

http://www.nature.com/nature/journal/v491/n7422/full/nature11433.html

 

本站声明: 生物文库所有文章欢迎转载,所有文章未说明,均属于原创,转载均请注明出处。
本文链接: http://www.bioku.cn/201212/nature-codon-usage-based-inhibition-human-schlafen11-hiv-protein-synthesis/
版权所有: 生物文库 - 生物医学、生物技术核心期刊文摘

留言


+ 七 = 11


沪ICP备12028140号
点击这里给我发消息   点击这里给我发消息