瑞士研究人员在美国同事们的帮助下,利用小鼠胚胎干细胞成功地制造出甲状腺组织。他们还发现将这种新产生的组织移植到10只实验小鼠的发生功能故障的甲状腺之上,能够让它们中的9只小鼠甲状腺功能完全恢复。

-2012年11月1日《自然》

中文翻译


【题目】胚胎干细胞功能性甲状腺的产生

【译文】甲状腺的主要功能是通过合成甲状腺激素类来促进碘化物代谢,它们是几乎所有组织中生长、发育和代谢的重要调节因子。到目前为止,对甲状腺形态发生的研究缺少一种有效的干细胞模型系统,即可以在体外模拟调控甲状腺卵泡细胞分化和后续组装成功能化甲状腺滤泡的分子和形态学事件的系统。本研究报道了当用促甲状腺激素处理时,转录因子NKX2-1和PAX8的瞬时过表达足以引发鼠胚胎干细胞分化成甲状腺滤泡细胞,而甲状腺滤泡细胞可以组成滤泡三维结构。这些体外来源的滤泡表现出碘化物有机化活性。重要的是,当移植入甲状腺功能减退小鼠中时,这些滤泡恢复了甲状腺激素血浆水平并促进后续症状的康复。因此,鼠胚胎干细胞可在体外被诱导分化成甲状腺滤泡细胞并形成功能性甲状腺组织。

英文原稿


[Title]: Generation of functional thyroid from embryonic stem cells

[Authors]:Francesco Antonica,1 Dominika Figini Kasprzyk,1 Robert Opitz,1 Michelina Iacovino,2 Xiao-Hui Liao,3 Alexandra Mihaela Dumitrescu,3 Samuel Refetoff,3, 4 Kathelijne Peremans,5 Mario Manto,6 Michael Kyba2 & Sabine Costagliola1

[Abstract]:The primary function of the thyroid gland is to metabolize iodide by synthesizing thyroid hormones, which are critical regulators of growth, development and metabolism in almost all tissues. So far, research on thyroid morphogenesis has been missing an efficient stem-cell model system that allows for the in vitro recapitulation of the molecular and morphogenic events regulating thyroid follicular-cell differentiation and subsequent assembly into functional thyroid follicles. Here we report that a transient overexpression of the transcription factors NKX2-1 and PAX8 is sufficient to direct mouse embryonic stem-cell differentiation into thyroid follicular cells that organize into three-dimensional follicular structures when treated with thyrotropin. These in vitro-derived follicles showed appreciable iodide organification activity. Importantly, when grafted in vivo into athyroid mice, these follicles rescued thyroid hormone plasma levels and promoted subsequent symptomatic recovery. Thus, mouse embryonic stem cells can be induced to differentiate into thyroid follicular cells in vitro and generate functional thyroid tissue.

原文地址

http://www.nature.com/nature/journal/v491/n7422/full/nature11525.html

 

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