science20121116-1

来自德国慕尼黑工业大学和美国密歇根大学的研究人员使用DNA纳米技术构建出一种人工膜通道,这种膜通道能够表现出门控响应,并可用于探测单分子。这一研究可为未来分子传感器、抗菌制剂等的开发提供技术支持。

-2012年11月16日《科学》

中文翻译


【题目】使用人工设计的DNA纳米结构构建脂膜通道

【译文】依靠自我组装的DNA纳米材料,我们在脂双层上构建出纳米级别的跨膜通道。我们使用支架DNA折纸技术,构建能够穿透和跨越脂膜的茎,以及附着到膜上的桶状帽子,这一过程部分通过26个胆固醇来完成。测定单通道的电生理学参数时,我们发现这种茎具有类似自然离子通道的响应,如类似大约1纳西门子的电导率和通道门的特点。当茎的单个DNA链插进通道时,会出现更加明显的门控变化。单分子移动实验证实人工膜通道可用于区分单个DNA分子。

英文原稿


[Title]: Synthetic Lipid Membrane Channels Formed by Designed DNA Nanostructures   

[Authors]: Martin Langecker, Vera Arnaut, Thomas G. Martin, Jonathan List, Stephan Renner, Michael Mayer, Hendrik Dietz, and Friedrich C. Simmel

[Abstract]: We created nanometer-scale transmembrane channels in lipid bilayers by means of self-assembled DNA-based nanostructures. Scaffolded DNA origami was used to create a stem that penetrated and spanned a lipid membrane, as well as a barrel-shaped cap that adhered to the membrane, in part via 26 cholesterol moieties. In single-channel electrophysiological measurements, we found similarities to the response of natural ion channels, such as conductances on the order of 1 nanosiemens and channel gating. More pronounced gating was seen for mutations in which a single DNA strand of the stem protruded into the channel. Single-molecule translocation experiments show that the synthetic channels can be used to discriminate single DNA molecules.

原文地址

http://www.sciencemag.org/content/338/6109/932.abstract

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