来自日本京都大学的研究人员使用小鼠的多能干细胞,成功获得了成熟的、功能发育完全的雌配子,并得到健康的幼鼠。这项工作为破解哺乳动物发育及不育症这一难题提供了强有力的技术支持。

-2012年11月16日《科学》

中文翻译


【题目】从干细胞制备有功能的人造小鼠卵子

 【译文】雌性生殖细胞的体外重建是生殖生物学和医学的重要挑战。本研究发现小鼠的雌性(XX)胚胎干细胞和诱导多能干细胞能够被诱导分化为原始生殖细胞样细胞(PGCLCs),与雌性性腺体细胞共同培养后,得到“再建的卵巢”,再经过X染色体再活化、印记擦除和囊肿形成,“再建的卵巢”中的细胞表现出减数分裂潜能。移植入小鼠的卵巢囊后,“再建的卵巢”中的PGCLCs成熟后分化为胚泡期卵母细胞,经过体外成熟和受精后形成大量的后代细胞。我们的培养体系可为研究雌性生殖细胞的关键特性(包括全能性获得)提供强有力的物质基础,还可用于体外雌性生殖细胞发育的重建。

英文原稿


[Title]: Offspring from Oocytes Derived from in Vitro Primordial Germ Cell–like Cells in Mice

[Authors]: Katsuhiko Hayashi, Sugako Ogushi, Kazuki Kurimoto, So Shimamoto, Hiroshi Ohta, and Mitinori Saitou

[Abstract]: Reconstitution of female germ cell development in vitro is a key challenge in reproductive biology and medicine. We show here that female (XX) embryonic stem cells and induced pluripotent stem cells in mice are induced into primordial germ cell–like cells (PGCLCs), which, when aggregated with female gonadal somatic cells as reconstituted ovaries, undergo X-reactivation, imprint erasure, and cyst formation, and exhibit meiotic potential. Upon transplantation under mouse ovarian bursa, PGCLCs in the reconstituted ovaries mature into germinal vesicle-stage oocytes, which then contribute to fertile offspring after in vitro maturation and fertilization. Our culture system serves as a robust foundation for the investigation of key properties of female germ cells, including the acquisition of totipotency, and for the reconstitution of whole female germ cell development in vitro.

原文地址

http://www.sciencemag.org/content/338/6109/971.short

本站声明: 生物文库所有文章欢迎转载,所有文章未说明,均属于原创,转载均请注明出处。
本文链接: http://www.bioku.cn/201212/science-reconstitute-female-germ-cell-in-vitro-induced-pluripotent-stem/
版权所有: 生物文库 - 生物医学、生物技术核心期刊文摘

留言


× 1 = 四


沪ICP备12028140号
点击这里给我发消息   点击这里给我发消息