cell20121121-17

来自美国叶史瓦大学阿尔伯特·爱因斯坦医学院遗传系的Bin Zhou实验室发现心内膜细胞通过血管发出的心肌到心内膜的VEGF信号形成冠状动脉,这篇文章刊登在最新一期《CELL》杂志上。

-2012年11月21日《细胞》


中文翻译


【题目】心内膜细胞通过血管发出的心肌到心内膜的VEGF信号形成冠状动脉

【译文】冠状动脉的起源和发育机制还没有被完全理解。我们通过囊胚发肓图、纯系分析和免疫组织化学的方法发现心内细胞可以形成冠状动脉的内皮组织。染色追踪、活体成像和组织移植都揭示了心室的内心膜细胞不能终末分化;相反的是,它们是具有血管原性的并且可以形成冠状内皮网络。心肌Vegf-a -a或心内膜Vegfr-2的缺失可以通过心内膜细胞抑制冠状血管发生和动脉形成。相反的是,谱系和敲除研究的结果显示心内膜细胞对于冠状静脉的形成具有小的促进作用,而冠状静脉的形成是不依赖于心肌到心内膜的Vegf信号的。因此,与现在关于冠状血管的一些观点相反的是,我们发现冠状动脉和静脉具有不同的起源并且形成的机制也不同。我们的这些发现可能可以为治疗冠状动脉疾病提供更好的细胞治疗策略。

英文原稿


[Title]Endocardial Cells Form the Coronary Arteries by Angiogenesis through Myocardial-Endocardial VEGF Signaling

[author]Bingruo Wu, Zheng Zhang, Wendy Lui, Xiangjian Chen, Yidong Wang, Alyssa A. Chamberlain, Ricardo A. Moreno-Rodriguez, Roger R. Markwald, Brian P. O’Rourke, David J. Sharp, Deyou Zheng, Jack Lenz, H. Scott Baldwin, Ching-Pin Chang, Bin Zhou

[Abstract]The origins and developmental mechanisms of coronary arteries are incompletely understood. We show here by fate mapping, clonal analysis, and immunohistochemistry that endocardial cells generate the endothelium of coronary arteries. Dye tracking, live imaging, and tissue transplantation also revealed that ventricular endocardial cells are not terminally differentiated; instead, they are angiogenic and form coronary endothelial networks. Myocardial Vegf-a or endocardial Vegfr-2 deletion inhibited coronary angiogenesis and arterial formation by ventricular endocardial cells. In contrast, lineage and knockout studies showed that endocardial cells make a small contribution to the coronary veins, the formation of which is independent of myocardial-to-endocardial Vegf signaling. Thus, contrary to the current view of a common source for the coronary vessels, our findings indicate that the coronary arteries and veins have distinct origins and are formed by different mechanisms. This information may help develop better cell therapies for coronary artery disease.

原文地址

http://www.cell.com/abstract/S0092-8674(12)01243-3

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