来自美国马萨诸塞州医学院的William E. Theurkauf实验室发现UAP56将piRNA簇和核周转座子沉默机制偶联在一起。

-2012年11月9日《细胞》


中文翻译


【题目】UAP56将piRNA簇和核周转座子沉默机制偶联在一起

【译文】在种系发育过程中piRNAs沉默转座子。在果蝇属中,来自异染色质簇的转录物在核周生殖云中被加工形成初始piRNAs。核DEAD盒蛋白UAP56之前被认为参与mRNA剪接和输出,但是DEAD盒蛋白Vasa在piRNA生产中具有确定的作用,且这些蛋白被定位于结合了piRNA和PIWI蛋白Ago3和Aub的生殖云。我们发现UAP56共定位于簇相关的HP1变体Rhino中,包含了Vasa的生殖云颗粒通过来自于包含了UAP56和Rhino位点的核包膜直接集中于一块区域,我们还发现簇转录物的免疫共沉淀包含了Vasa和UAP56。重要的是,电荷取代突变改变了UAP56中的一个保守的表面残基,这种突变破坏了Rhino、piRNA产生、转座子沉默和Vasa核周定位的共定位。因此我们认为UAP56和Vasa的功能是分隔核包膜。

英文原稿


[Title]UAP56 Couples piRNA Clusters to the Perinuclear Transposon Silencing Machinery

[Authors]Fan Zhang, Jie Wang, Jia Xu, Zhao Zhang, Birgit S. Koppetsch, Nadine Schultz, Thom Vreven, Carine Meignin, Ilan Davis, Phillip D. Zamore, Zhiping Weng, William E. Theurkauf

[Abstract]piRNAs silence transposons during germline development. In Drosophila, transcripts from heterochromatic clusters are processed into primary piRNAs in the perinuclear nuage. The nuclear DEAD box protein UAP56 has been previously implicated in mRNA splicing and export, whereas the DEAD box protein Vasa has an established role in piRNA production and localizes to nuage with the piRNA binding PIWI proteins Ago3 and Aub. We show that UAP56 colocalizes with the cluster-associated HP1 variant Rhino, that nuage granules containing Vasa localize directly across the nuclear envelope from cluster foci containing UAP56 and Rhino, and that cluster transcripts immunoprecipitate with both Vasa and UAP56. Significantly, a charge-substitution mutation that alters a conserved surface residue in UAP56 disrupts colocalization with Rhino, germline piRNA production, transposon silencing, and perinuclear localization of Vasa. We therefore propose that UAP56 and Vasa function in a piRNA-processing compartment that spans the nuclear envelope.

原文地址

http://www.cell.com/abstract/S0092-8674(12)01239-1

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