nature20121213-7

美国密苏里大学的研究人员发现了一件令人惊讶的事情,抗虫大豆中的其中一种抗胞囊线虫的基因与包括淋巴细胞性白血病、脊柱裂和心血管疾病在内的一些人类疾病的发生有关。

-2012年12月13日《自然》


中文翻译


【题目】大豆胞囊线虫耐受基因指出植物对病原体耐受的新机制

【译文】 大豆是一种重要的作物,为全球提供了蛋白和油的可持续资源。大豆胞囊线虫是一种微小线虫,以大豆根部为食,是大豆产量的主要限制因素。这种线虫单在美国平均每年会造成超过10亿美元的损失,这使其成为大豆中经济上最重要的病原生物。尽管耐受品种的种植形成了对付这种病原生物的核心应对策略,但对耐受性的本质还知之甚少。此外,对于多数已知耐受资源来说,这种寄生虫毒性群体的增加迫使我们急需开发控制的新方法。本研究报道了Rhg4座位上一个基因的基于图谱的克隆,该座位是有助于对这种病原微生物耐受的一种主要数量性状遗传位点。突变分析、基因沉默和转基因互补证实了该基因缺失承担了耐受。该基因编码丝氨酸羟甲基转移酶,这是一种在自然界普遍存在的酶,其结构保守。 该酶负责丝氨酸和甘氨酸的互变,并对于细胞一碳代谢至关重要。负责耐受或敏感的Rhg4等位基因可通过两种遗传多态性区分,这种遗传多态性改变了该酶的关键调控特性。我们的发现揭示了先前未知的植物对一种病原体的耐受机制。对耐受基因的机制学理解可被开发以改善全球重要作物大豆对线虫的耐受性。

英文原稿


[Title]: A soybean cyst nematode resistance gene points to a new mechanism of plant resistance to pathogens

[Authors]:Shiming Liu,1, 6 Pramod K. Kandoth,2, 6 Samantha D. Warren,3 Greg Yeckel,2 Robert Heinz,2 John Alden,2 Chunling Yang,4 Aziz Jamai,1 Tarik El-Mellouki,1 Parijat S. Juvale,4 John Hill,4 Thomas J. Baum,4 Silvia Cianzio,5 Steven A. Whitham,4 Dmitry Korkin,3 Melissa G. Mitchum2 & Khalid Meksem1

[Abstract]Soybean (Glycine max (L.) Merr.) is an important crop that provides a sustainable source of protein and oil worldwide. Soybean cyst nematode (Heterodera glycines Ichinohe) is a microscopic roundworm that feeds on the roots of soybean and is a major constraint to soybean production. This nematode causes more than US$1 billion in yield losses annually in theUnited States alone, making it the most economically important pathogen on soybean. Although planting of resistant cultivars forms the core management strategy for this pathogen, nothing is known about the nature of resistance. Moreover, the increase in virulent populations of this parasite on most known resistance sources necessitates the development of novel approaches for control. Here we report the map-based cloning of a gene at the Rhg4 (for resistance to Heterodera glycines 4) locus, a major quantitative trait locus contributing to resistance to this pathogen. Mutation analysis, gene silencing and transgenic complementation confirm that the gene confers resistance. The gene encodes a serine hydroxymethyltransferase, an enzyme that is ubiquitous in nature and structurally conserved across kingdoms. The enzyme is responsible for interconversion of serine and glycine and is essential for cellular one-carbon metabolism. Alleles of Rhg4 conferring resistance or susceptibility differ by two genetic polymorphisms that alter a key regulatory property of the enzyme. Our discovery reveals an unprecedented plant resistance mechanism against a pathogen. The mechanistic knowledge of the resistance gene can be readily exploited to improve nematode resistance of soybean, an increasingly important global crop.

原文地址

http://www.nature.com/nature/journal/v492/n7428/full/nature11651.html

 

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