nature-medicine201212-10

来自美国麻省总医院的科学家日前开发了一种整合了NMR检测系统的微芯片,用于快速分析直接来源于成胶质细胞瘤患者血样本的循环微泡。

-2012年12月《自然-医学》


中文翻译


【题目】循环微泡的蛋白分型使得实时监测成胶质细胞瘤治疗过程变为可能

【译文】成胶质细胞瘤产生大量小的、膜结合微泡,这些微泡随后进入循环系统。尽管这些微泡有希望成为治疗反应的潜在生物标志物,但它们的鉴定和定量化还存在挑战。本研究描述了分析直接从成胶质细胞瘤血样本中循环微泡的一种高度敏感和快速分析技术。微泡,引进至专用的微芯片中,用靶点特异性磁性毫微粒加以标记,并可通过一种小型核磁共振系统加以检测。与当前方法比较,该集成系统具有更高的检测灵敏度,并可以从非瘤宿主细胞来源的微泡中区分出成多形性胶质细胞瘤(GBM)微泡。我们的研究还表明循环GBM微泡可用于分析原发性肿瘤突变,并作为治疗诱导变化的可预测度量标准。该平台可能为人类临床试验提供了药物效用的早期指示剂和潜在分子分层。

英文原稿


[Title]: Protein typing of circulating microvesicles allows real-time monitoring of glioblastoma therapy

[Authors]:Huilin Shao,1, 2 Jaehoon Chung,1 Leonora Balaj,3 Alain Charest,4 Darell D Bigner,5 Bob S Carter,6 Fred H Hochberg,7 Xandra O Breakefield,3, 8 Ralph Weissleder1, 7, 9, 10 & Hakho Lee1, 10

[Abstract]Glioblastomas shed large quantities of small, membrane-bound microvesicles into the circulation. Although these hold promise as potential biomarkers of therapeutic response, their identification and quantification remain challenging. Here, we describe a highly sensitive and rapid analytical technique for profiling circulating microvesicles directly from blood samples of patients with glioblastoma. Microvesicles, introduced onto a dedicated microfluidic chip, are labeled with target-specific magnetic nanoparticles and detected by a miniaturized nuclear magnetic resonance system. Compared with current methods, this integrated system has a much higher detection sensitivity and can differentiate glioblastoma multiforme (GBM) microvesicles from nontumor host cell–derived microvesicles. We also show that circulating GBM microvesicles can be used to analyze primary tumor mutations and as a predictive metric of treatment-induced changes. This platform could provide both an early indicator of drug efficacy and a potential molecular stratifier for human clinical trials.

原文地址

http://www.nature.com/nm/journal/v18/n12/abs/nm.2994.html

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