nature-medicine201212-11

美国斯坦福大学的科学家开发出一种荧光成像技术,能够使活体动物血管脉动以前所未有的清晰度呈现。与传统的影像技术相比,其增加的清晰度类似于擦拭掉眼镜前的迷雾一般。

-2012年12月《自然-医学》


中文翻译


【题目】利用近红外II荧光的体内多功能血管成像技术

【译文】二级近红外区(NIR-II)小鼠后肢脉管系统的体内实时荧光成像是通过单壁碳纳米管作为荧光基团实现的。由于提供深度解破学传统和低散射的有利NIR- II光学窗口,小血管成像的高空间(~30 μm)和时间(<200 ms)分辨率在后肢中1-3mm深度实现。这种空间分辨率通过NIR成像技术(NIR-I)或显微计算断层技术难以实现,而时间分辨率远远超过扫描显微成像技术。利用一种动态对照增强的NIR-II成像技术,以不同血流动力学为基础,可以清晰地区分动脉和静脉血管。而且,深度组织穿透力和NIR- II成像的高空间及时间分辨率使得正常和局部缺血的股动脉中血流速率准确定量变为可能,且在检测较低血流的速率上强于超声检查法。

英文原稿


[Title]: Multifunctional in vivo vascular imaging using near-infrared II fluorescence

[Authors]:Guosong Hong,1, 3 Jerry C Lee,2, 3 Joshua T Robinson,1 Uwe Raaz,2 Liming Xie,1 Ngan F Huang,2 John P Cooke2 & Hongjie Dai1

[Abstract]In vivo real-time epifluorescence imaging of mouse hind limb vasculatures in the second near-infrared region (NIR-II) is performed using single-walled carbon nanotubes as fluorophores. Both high spatial (~30 μm) and temporal (<200 ms per frame) resolution for small-vessel imaging are achieved at 1–3 mm deep in the hind limb owing to the beneficial NIR-II optical window that affords deep anatomical penetration and low scattering. This spatial resolution is unattainable by traditional NIR imaging (NIR-I) or microscopic computed tomography, and the temporal resolution far exceeds scanning microscopic imaging techniques. Arterial and venous vessels are unambiguously differentiated using a dynamic contrast-enhanced NIR-II imaging technique on the basis of their distinct hemodynamics. Further, the deep tissue penetration and high spatial and temporal resolution of NIR-II imaging allow for precise quantifications of blood velocity in both normal and ischemic femoral arteries, which are beyond the capabilities of ultrasonography at lower blood velocities.

原文地址

http://www.nature.com/nm/journal/v18/n12/abs/nm.2995.html

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