nature20121220-10

来自德国慕尼黑-路德维希-马克西米连大学的科学家日前报道了来自酿酒酵母中介体头部模块的晶体结构。

-2012年12月20日《自然》


中文翻译


【题目】中介体头部模块的结构

【译文】RNA聚合酶(Pol) II介导的基因转录需要共激活体复合物中介体。 中介体连接转录调控因子和Pol II,并与人类疾病相关。酿酒酵母中的中介体的分子量为1.4兆道尔顿,含有形成头部、中部和激酶模块的25个亚基。头部模块组成了一半重要介导体核心,而且包含了保守亚单位Med6、Med8、Med11、Med17、Med18、Med20和Med22。近期对酿酒酵母头部模块的分辨率为4.3 Å 的X射线分析表明了具有三个亚模块(称为颈部、固定的下巴及移动下巴)的部分结构模式。本研究解析了来自粟酒裂殖酵母的头部晶体结构,分辨率为3.4Å。Med6和固定下巴的新结构以及移动下巴和颈部部分先前的结构使得结构解析变为可能,并需要Med20的缺失。粟酒裂殖酵母头部模块类似于具有8种不同元件的鳄鱼头部,其中四种元件是可移动的。固定的下巴包含牙齿和鼻子结构域,而颈部亚模块包含一种螺旋棘和肢,具有肩膀、手臂和手指元件。手臂和必要肩膀包含中介体的其他部分。下巴和中枢连接与Pol II及其碳末端结构域相互作用相关,而且体内连接对于转录是必需的。粟酒裂殖酵母头部模块结构形成了酿酒酵母模块的校正模型,揭示了高度保守性和柔性,解释了已知的突变,并为阐释基因调控的核心机制提供了基础。

英文原稿


[Title]: Structure of the Mediator head module

[Authors]:Laurent Larivière,1, 2 Clemens Plaschka,1, 2 Martin Seizl,1 Larissa Wenzeck,1 Fabian Kurth1, 3 & Patrick Cramer1

[Abstract]:Gene transcription by RNA polymerase (Pol) II requires the coactivator complex Mediator. Mediator connects transcriptional regulators and Pol II, and is linked to human disease. Mediator from the yeast Saccharomyces cerevisiae has a molecular mass of 1.4 megadaltons and comprises 25 subunits that form the head, middle, tail and kinase modules. The head module constitutes one-half of the essential Mediator core, and comprises the conserved subunits Med6, Med8, Med11, Med17, Med18, Med20 and Med22. Recent X-ray analysis of the S. cerevisiae head module at 4.3Å resolution led to a partial architectural model with three submodules called neck, fixed jaw and moveable jaw. Here we determine de novo the crystal structure of the head module from the fission yeast Schizosaccharomyces pombe at 3.4Å resolution. Structure solution was enabled by new structures of Med6 and the fixed jaw, and previous structures of the moveable jaw and part of the neck, and required deletion of Med20. The S. pombe head module resembles the head of a crocodile with eight distinct elements, of which at least four are mobile. The fixed jaw comprises tooth and nose domains, whereas the neck submodule contains a helical spine and one limb, with shoulder, arm and finger elements. The arm and the essential shoulder contact other parts of Mediator. The jaws and a central joint are implicated in interactions with Pol II and its carboxy-terminal domain, and the joint is required for transcription in vitro. The S. pombe head module structure leads to a revised model of the S. cerevisiae module, reveals a high conservation and flexibility, explains known mutations, and provides the basis for unravelling a central mechanism of gene regulation.

原文地址

http://www.nature.com/nature/journal/v492/n7429/full/nature11670.html

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