来自德国雷根斯堡大学的研究人员发现拟南芥富含半胱氨酸的EC1蛋白能够调控卵细胞和中央细胞各自与精细胞融合,从而完成双受精作用。

-2012年11月23日《科学》

中文翻译


【题目】卵细胞分泌的EC1蛋白在双受精过程中引发激活精细胞

【译文】双受精作用是开花植物所特有的现象。然而,调控第一个精子与卵细胞、第二个精子与中央细胞融合的分子机制还不清楚。本研究发现拟南芥中的配子互作依赖小的、富含半胱氨酸的EC1(EGG CELL 1)蛋白在卵细胞储藏囊泡中的积累量。一旦精子到达卵细胞表面,包含EC1蛋白的囊泡被分泌到胞外。精子的内膜系统能够响应外源添加的EC1蛋白,将具潜能的配子融合剂HAP2/GCS1(HAPLESS 2/GENERATIVE CELL SPECIFIC 1)蛋白重新分布在细胞表面。此外,对ec1基因五倍体突变植株的受精作用研究,本研究成功地证明雌-雄配子的互作是阻止多个精细胞进入同一雌配子的关键。我们的发现证实配子的共同激活、受调控的胞外分泌和精子质膜的修饰共同控制开花植物的配子互作。

英文原稿


[Title]: Egg Cell–Secreted EC1 Triggers Sperm Cell Activation During Double Fertilization

[Authors]:Stefanie Sprunck, Svenja Rademacher, Frank Vogler, Jacqueline Gheyselinck, Ueli Grossniklaus, and Thomas Dresselhaus

[Abstract]: Double fertilization is the defining characteristic of flowering plants. However, the molecular mechanisms regulating the fusion of one sperm with the egg and the second sperm with the central cell are largely unknown. We show that gamete interactions in Arabidopsis depend on small cysteine-rich EC1 (EGG CELL 1) proteins accumulating in storage vesicles of the egg cell. Upon sperm arrival, EC1-containing vesicles are exocytosed. The sperm endomembrane system responds to exogenously applied EC1 peptides by redistributing the potential gamete fusogen HAP2/GCS1 (HAPLESS 2/GENERATIVE CELL SPECIFIC 1) to the cell surface. Furthermore, fertilization studies with ec1 quintuple mutants show that successful male-female gamete interactions are necessary to prevent multiple–sperm cell delivery. Our findings provide evidence that mutual gamete activation, regulated exocytosis, and sperm plasma membrane modifications govern flowering plant gamete interactions.

原文地址

http://www.sciencemag.org/content/338/6110/1093.short

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