来自美国加州Salk研究所的研究人员发现鼠科动物的脑肿瘤不一定需要来源于神经干细胞,也可以来源于成熟的神经细胞和星形胶质细胞。

-2012年11月23日《科学》

中文翻译


【题目】致癌基因引起细胞去分化进而诱导小鼠发生神经胶质瘤

【译文】多形性胶质母细胞瘤(GBM)是人类最常见的、具有侵袭性的恶性原发性脑肿瘤。本研究发现神经胶质瘤能够来源于在中枢神经系统(CNS)中已分化的细胞,如皮层神经元。研究发现若向小鼠大脑中的神经干细胞(NSCs)、星形胶质细胞甚至是成熟的神经细胞导入致癌性的慢病毒载体,会导致小鼠出现恶性神经胶质瘤。所有的肿瘤,不论慢病毒载体的注射位点(起始细胞群),具有的共同特征为:高表达水平的干细胞或祖细胞标志物和低表达水平的分化标志物。基因芯片分析揭示星形细胞和神经细胞的肿瘤起源于间充质的GBM亚型。本研究认为中枢神经系统中大多数已分化的细胞,根据已确定遗传改造程序,经历去分化过程形成神经干细胞或祖细胞,进而起始和维持肿瘤发展,以及引起恶性神经胶质瘤中观察到的异质种群。

英文原稿


[Title]: Dedifferentiation of Neurons and Astrocytes by Oncogenes Can Induce Gliomas in Mice   

[Authors]:Dinorah Friedmann-Morvinski, Eric A. Bushong, Eugene Ke, Yasushi Soda, Tomotoshi Marumoto, Oded Singer, Mark H. Ellisman, and Inder M. Verma

[Abstract]: Glioblastoma multiforme (GBM) is the most common and aggressive malignant primary brain tumor in humans. Here we show that gliomas can originate from differentiated cells in the central nervous system (CNS), including cortical neurons. Transduction by oncogenic lentiviral vectors of neural stem cells (NSCs), astrocytes, or even mature neurons in the brains of mice can give rise to malignant gliomas. All the tumors, irrespective of the site of lentiviral vector injection (the initiating population), shared common features of high expression of stem or progenitor markers and low expression of differentiation markers. Microarray analysis revealed that tumors of astrocytic and neuronal origin match the mesenchymal GBM subtype. We propose that most differentiated cells in the CNS upon defined genetic alterations undergo dedifferentiation to generate a NSC or progenitor state to initiate and maintain the tumor progression, as well as to give rise to the heterogeneous populations observed in malignant gliomas.

原文地址

http://www.sciencemag.org/content/338/6110/1080.short

 

本站声明: 生物文库所有文章欢迎转载,所有文章未说明,均属于原创,转载均请注明出处。
本文链接: http://www.bioku.cn/201302/science-gbm-lentiviral-vector-dedifferentiation-astrocyte-nsc/
版权所有: 生物文库 - 生物医学、生物技术核心期刊文摘

留言


四 × 5 =


沪ICP备12028140号
点击这里给我发消息   点击这里给我发消息