nature20130117-9

哈佛干细胞研究所等处研究人员揭示了哺乳动物心肌细胞再生来源于预存心肌细胞。

-2013年1月17日《自然》

中文翻译


【题目】成年哺乳动物心肌细胞更新源自现有细胞而非干细胞

【译文】近来虽有一些研究表明,成年哺乳动物心脏细胞可再生,但细胞再生频率和新心脏细胞来源尚不清楚。一些研究表明,干细胞具有很高的分化成心肌祖细胞的活性。其他的研究表明,新的心肌细胞再生速率很低,并且它们来源于现有心肌细胞的细胞分裂。通过结合两种不同的脉冲追逐方法,即稳定同位素标记的遗传作图和质谱多同位素成像,本研究发现在正常老化过程中(逐渐向心肌损伤区域靠近),心肌细胞起源于现有的心肌细胞分裂,且再生速率很低。我们发现,正常细胞老化和损伤过程中,细胞周期活动导致多倍体、多核和二倍体单核心肌细胞的产生。这些研究数据表明现有心肌细胞是维持正常哺乳动物心肌平衡和心肌损伤中心肌细胞的主要来源。

英文原稿


[Title]: Mammalian heart renewal by pre-existing cardiomyocytes

[Authors]:Samuel E. Senyo,1 Matthew L. Steinhauser,1 Christie L. Pizzimenti,1 Vicky K. Yang,1 Lei Cai,1 Mei Wang,4, 5 Ting-Di Wu,2, 3 Jean-Luc Guerquin-Kern,2, 3 Claude P. Lechene4, 5 & Richard T. Lee1, 6

[Abstract]Although recent studies have revealed that heart cells are generated in adult mammals, the frequency of generation and the source of new heart cells are not yet known. Some studies suggest a high rate of stem cell activity with differentiation of progenitors to cardiomyocytes. Other studies suggest that new cardiomyocytes are born at a very low rate and that they may be derived from the division of pre-existing cardiomyocytes. Here we show, by combining two different pulse–chase approaches—genetic fate-mapping with stable isotope labelling, and multi-isotope imaging mass spectrometry—that the genesis of cardiomyocytes occurs at a low rate by the division of pre-existing cardiomyocytes during normal ageing, a process that increases adjacent to areas of myocardial injury. We found that cell cycle activity during normal ageing and after injury led to polyploidy and multinucleation, but also to new diploid, mononucleate cardiomyocytes. These data reveal pre-existing cardiomyocytes as the dominant source of cardiomyocyte replacement in normal mammalian myocardial homeostasis as well as after myocardial injury.

原文地址

http://www.nature.com/nature/journal/v493/n7432/full/nature11682.html

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